One-Step Reverse Transcription PCR Reagents
ABclonal Technology AFTSpin EndoFree Plasmid Mini Kit
This kit uses a modified SDS-alkaline lysis method to lyse cells. Crude extract is selectively bound to the spin column by a unique endotoxin scavenger and centrifuged to remove endotoxin. The silicone matrix membrane in the spin column selectively binds plasmid DNA at high salt and low pH conditions, allowing for impurities and other bacterial components to be removed by Buffer PR and Buffer WB. The purified plasmid DNA is eluted from the silicone matrix membrane using low-salt and high-pH Buffer EB.
ABclonal Technology AFTSpin Multifunction DNA Purification Kit
The AFTSpin Multifunction DNA Purification Kit enables rapid gel recovery and PCR product purification using silica-column technology. DNA binds to the membrane in high-salt conditions, while impurities are removed via wash steps. A unique gel lysis/binding solution (yellow for easy pH monitoring) combines gel digestion and DNA binding, streamlining agarose recovery, PCR cleanup, and enzyme digestion purification. Delivers high-purity DNA with improved efficiency in a single kit.
ABclonal Technology Fast One Step Probe RT-qPCR Kit
The Fast One Step Probe RT-qPCR Kit is a reagent kit designed for rapid amplification quantitative RT-qPCR detection using the probe method. It includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, and heat-labile UDG. This kit utilizes RNA as a template and includes gene-specific primers, enabling consecutive reverse transcription and PCR reactions to take place in the same tube without the requirement for additional pipetting or tube opening steps. This streamlined process significantly enhances throughput. The kit utilizes dUTP/UDG to prevent contamination, with the heat-sensitive UDG rapidly degrading uracil-containing contaminants at room temperature. At 50°C during reverse transcription, the heat-labile UDG quickly deactivates, ensuring it does not affect the efficiency and sensitivity of RT-qPCR.
VAZYME INTERNATIONAL LLC Vazyme International AccurSTART II U+ One Step RT-qPCR Super Premix, Fast, One Tube
This preassembled, probe-based master mix is engineered for one-tube, one-step RT-qPCR applications. It is optimized for rapid and sensitive quantification of RNA templates in both singleplex and multiplex assays, such as for RNA viruses.
- Fully preassembled and stable, compatible with preloaded primers and probes, ideal for long-term storage.
- Ultra-fast amplification with reliable detection within 22 minutes, maintaining sensitivity.
- Direct amplification compatibility, supporting high-volume input of crude lysates without purification.
- High-sensitivity multiplexing for accurate detection of low-copy viral RNA across multiple targets.
- Engineered hot-start enzyme system ensures precise activation and minimizes non-specific amplification.
- dUTP/UDG contamination control system functions at room temperature, preventing carryover.
Meridian Life Science Lyo-Ready 1-Step RT-qPCR ROX, 100ML
Lyo-Ready 1-Step RT-qPCR ROX Mix, 4x is a glycerol-free, one tube formulation combining the latest advances in buffer chemistry and PCR enhancers, together with an optimized concentration of reverse transcriptase, antibody-mediated hot-start polymerase, dNTPs and MgCl2. Lyo-Ready 1-Step RT-qPCR ROX Mix has been designed for highly reproducible, accurate RNA and DNA target amplification, delivering excellent results in multiplex assays. In order to produce room temperature lyophilized RT-qPCR reagents, assay specific primers and probes can be added to Lyo-Ready 1-Step RT-qPCR ROX Mix for subsequent lyophilization.
Meridian Life Science Lyo-Ready 1-Step RT-qPCR ROX, 10ML
Lyo-Ready 1-Step RT-qPCR ROX Mix, 4x is a glycerol-free, one tube formulation combining the latest advances in buffer chemistry and PCR enhancers, together with an optimized concentration of reverse transcriptase, antibody-mediated hot-start polymerase, dNTPs and MgCl2. Lyo-Ready 1-Step RT-qPCR ROX Mix has been designed for highly reproducible, accurate RNA and DNA target amplification, delivering excellent results in multiplex assays. In order to produce room temperature lyophilized RT-qPCR reagents, assay specific primers and probes can be added to Lyo-Ready 1-Step RT-qPCR ROX Mix for subsequent lyophilization.
Cytek Biosciences Inc Classic++™ 1-Step RT-PCR Kit
Classic++™ 1-Step RT-PCR Kit ..... Cat. No. 31-5220-0050R ..... Size 50 rxns
Meridian Life Science Fast 1-Step RT-qPCR Reaction, 100ML
Fast 1-Step RT-qPCR Reaction Mix, 2x has been designed with the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a fast hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate, sensitive RT-qPCR efficiency in a single tube, even from low abundance targets and scarce samples. With the addition of reverse transcriptase and RNase Inhibitor, it delivers excellent results in both singleplex and multiplex RT-qPCR assays and confers superior assay performance under fast thermal cycling conditions.
Meridian Life Science Low LOD 1-Step RT-qPCR Mix, 1ML
Low LOD 1-Step RT-qPCR Mix comprises a master mix containing Taq polymerase, reaction buffer, MgCl2 and optimized dNTP/dUTP mix, and separate reverse transcriptase/RNase inhibitor mix. Low LOD 1-Step RT-qPCR Mix is designed for stringent amplification of highly-structured and very low copy-number RNA and DNA targets, ideal for applications such as blood bank or transplant viral testing.
Meridian Life Science Air-Dryable RT-LAMP 1-Step, 50ML
Air-Dryable RT-LAMP 1-Step Mix is a glycerol-free mix for isothermal applications such as Loop-Mediated Isothermal Amplification (LAMP). It contains Reverse Transcriptase, Bst-DNA Polymerase (exo-), reaction buffer, dNTP and provided with air-dryable excipients. This is a robust master mix for loop-mediated isothermal amplification (LAMP) reactions, has been designed for amplification of RNA and DNA targets. In order to produce dried, ambient temperature stable RT-LAMP assays, assay specific primers are added to Air-Dryable RNA/DNA LAMP for subsequent air-drying.
Meridian Life Science Lyo-Ready 1-Step RT-qPCR Mix, 1ML
Lyo-Ready 1-Step RT-qPCR Mix, 2x is a glycerol-free formulation that contains Taq polymerase, RNase Inhibitor, reaction buffer, dNTP, MgCl2 and lyo-excipients and separate reverse transcriptase and dilution buffer. It is ideal for developing room temperature, lyophilized, multiplex RT-qPCR tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.
Meridian Life Science Lyo-Ready 1-Step RT-qPCR, 1ML
Lyo-Ready 1-Step RT-qPCR Virus Mix can be used for the development of both RNA and DNA based molecular diagnostic tests, it contains a separate lyo-compatible thermostable reverse transcriptasex that has been optimized for amplification of RNA viruses with a high secondary structure (reverse transcriptase remains active at 55-60 C).